An elution technique for the estimation of dehydrogenase isoenzymes in agar gels.

The preparation described here is permanently cleared and water proof. It has the advantage of being fixed to a glass slide for easy handling and storage, yet it is adaptable for scanning (EEL ‘Scanner’). After staining the cellulose acetate electropherograms (Oxoid, OX0 Ltd.,) in Ponceau S, they are rinsed in 5% acetic acid and air dried. The strips (2.5 x IO cm) are trimmed such that each protein separation fits on a standard microscope slide (I x 3 in). The slide is first dipped in glacial acetic acid, allowing the excess to drip off, and then the cellulose acetate strip is placed on the wet slide with care being taken to remove all air bubbles. The slide with the strip attached is immersed in glacial acetic acid for 10-15 min or until the strip is completely cleared. It is then removed and air dried.